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). The protein-DNA interface entails in depth DNA contacts because of the NTase, OB, and HhH domains over a 19-bp section of duplex DNA centered regarding the nick (supplemental Fig. S3). The NTase domain binds to the damaged DNA strands at and flanking the nick; the OB domain contacts the continuous template strand bordering the nick; and the HhH area binds both equally strands throughout the small groove at the periphery of your footprint.
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Every action of the ligation pathway is dependent on a different subset on the LigA domains, with just the NTase domain currently being required for all methods. Area Ia is exclusive to NAD+-dependent ligases, is accountable for binding the NMN moiety of NAD+, and is needed for that response with NAD+ to variety the ligase-AMP intermediate (10, 11).
; with DNA omitted) had been superimposed Ligaz Ufabet Slots with respect for their NTase domains after which you can offset laterally to expose the big movement in the OB domain activated by nick recognition.
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